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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/21595
Title: Hemoglobin Pakse: Presence on red blood cell membrane and detection by polymerase chain reaction - Single-strand conformational polymorphism
Authors: Chairat Turbpaiboon
Atchasai Siritantikorn
Wanna Thongnoppakhun
Duangkamon Bunditworapoom
Chanin Limwongse
Pa Thai Yenchitsomanus
Noppadol Siritanaratkul
Prapon Wilairat
Mahidol University
Chulalongkorn University
Keywords: Medicine
Issue Date: 1-Aug-2004
Citation: International Journal of Hematology. Vol.80, No.2 (2004), 136-139
Abstract: Nondeletional gene mutations giving rise to α-thalassemia can be found at polymorphic frequency in Southeast Asia. Although the most common is hemoglobin Constant Spring (Hb CS), caused by a termination codon mutation (UAA → CAA, Gln) in the α2-globin gene and resulting in reduced synthesis of the elongated α-globin variant, Hb Pakse (UAA → UAU, Tyr) also has been observed at a significant prevalence. Western blot analysis of ghost membrane proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis from an individual with α-thal 1/Hb Pakse revealed the existence of a higher molecular weight globin of 18 kd consistent with an αPakse-globin chain. The presence of αPakse- globin on membranes of Hb Pakse-containing red blood cells affords an explanation for the severity of anemia observed in such patients. However, because the 2 Hb variants cannot be distinguished by current biochemical techniques, we developed a convenient single-tube polymerase chain reaction-single-strand conformational polymorphism (PCR-SSCP) protocol for the simultaneous diagnosis of Hb CS and Hb Pakse by amplifying a short fragment covering the termination codon of the α2-globin gene. This PCR-SSCP method required no internal control coamplification or use of restriction enzymes and has the potential of identifying all the other possible termination codon mutations in a single reaction with only 1 pair of primers. ©2004 The Japanese Society of Hematology.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=4444286637&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/21595
ISSN: 09255710
Appears in Collections:Scopus 2001-2005

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