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Title: Antiproliferation, antioxidation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line
Authors: Primchanien Moongkarndi
Nuttavut Kosem
Sineenart Kaslungka
Omboon Luanratana
Narongchai Pongpan
Neelobol Neungton
Mahidol University
Thailand Government Pharmaceutical Organization
Faculty of Medicine, Siriraj Hospital, Mahidol University
Keywords: Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Jan-2004
Citation: Journal of Ethnopharmacology. Vol.90, No.1 (2004), 161-166
Abstract: This study was designed to determine the antiproliferative, apoptotic and antioxidative properties of crude methanolic extract (CME) from the pericarp of Garcinia mangostana (family Guttiferae) using human breast cancer (SKBR3) cell line as a model system. SKBR3 cells were cultured in the presence of CME at various concentrations (0-50μg/ml) for 48h and the percentage of cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-di phenyl tetrazolium bromide (MTT) assay. CME showed a dose-dependent inhibition of cell proliferation with ED50of 9.25±0.64μg/ml. We found that antiproliferative effect of CME was associated with apoptosis on breast cancer cell line by determinations of morphological changes and oligonucleosomal DNA fragments. In addition, CME at various concentrations and incubation times were also found to inhibit ROS production. These investigations suggested that the methanolic extract from the pericarp of Garcinia mangostana had strong antiproliferation, potent antioxidation and induction of apoptosis. Thus, it indicates that this substance can show different activities and has potential for cancer chemoprevention which were dose dependent as well as exposure time dependent. © 2003 Elsevier Ireland Ltd. All rights reserved.
ISSN: 03788741
Appears in Collections:Scopus 2001-2005

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