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Title: Coupling morphogenesis to mitotic entry
Authors: Krisada Sakchaisri
Satoshi Asano
Li Rong Yu
Mark J. Shulewitz
Chong J. Park
Jung Eun Park
Young Wook Cho
Timothy D. Veenstra
Jeremy Thorner
Kyung S. Lee
National Cancer Institute
National Cancer Institute at Frederick
University of California, Berkeley
Mahidol University
Keywords: Multidisciplinary
Issue Date: 23-Mar-2004
Citation: Proceedings of the National Academy of Sciences of the United States of America. Vol.101, No.12 (2004), 4124-4129
Abstract: In eukaryotes, cyclin B-bound cyclin-dependent protein kinase 1 promotes mitotic entry but is held in check, in part, by Wee1 protein kinase. Timely mitotic entry in budding yeast requires inactivation of Swe1 (Wee1 ortholog). Perturbations of the septin collar at the bud neck lead to Swe1 stabilization, delaying the G2/M transition. Swe1 is recruited to the neck and hyperphosphorylated before ubiquitin-mediated degradation. Hsl1 kinase (Nim1 ortholog), a negative regulator of Wee1, is required for efficient Swe1 localization at the neck but seems not to phosphorylate Swe1. Here, we show that two other kinases targeted sequentially to the neck, Cla4/PAK and Cdc5/Polo, are responsible for stepwise phosphorylation and down-regulation of Swe1. This mechanism links assembly of a cellular structure to passage into mitosis.
ISSN: 00278424
Appears in Collections:Scopus 2001-2005

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