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Title: L-(<sup>3</sup>H) glutamate binding to a membrane preparation from the optic lobe of the giant freshwater prawn Macrobrachium rosenbergii de Man
Authors: Pisamai Pratumtan
Piyarat Govitrapong
Boonsirm Withyachumnarnkul
Boonserm Poolsanguan
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Medicine;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Jan-1991
Citation: Life Sciences. Vol.48, No.1 (1991), 19-26
Abstract: Membrane preparation from the optic lobe of the giant freshwater prawn, Macrobrachium rosenbergii de Man, was examined for the presence of specific L-(3H) glutamate binding. The optic lobes were isolated from live animals. The tissue was homogenized and the membrane fraction isolated by differential centrifugation. The membrane suspension was incubated with 10-1,000 nM of L-(3H) glutamate at 37 ° C for 60 min. Nonspecific binding was determined by incubating the mixture with 100 μM L-glutamate. L-(3H) glutamate specifically bound to the membrane fraction with a dissociation equilibrium constant (Kd) of 205 nM and maximum number of binding sites (Bmax) of 2.04 n mol/mg protein. By using LIGAND computerized program, the saturation isotherm binding pattern indicates a single type of binding. To determine the type of glutamate receptors, competitive inhibition and IC50of several glutamate agonists and antagonists were determined. The study reveals a metabotropic type of binding site. © 1991.
ISSN: 00243205
Appears in Collections:Scopus 1991-2000

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