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Title: Transformation of Aspergillus oryzae with a dominant selectable marker
Authors: S. Cheevadhanarak
G. Saunders
D. V. Renno
T. W. Flegel
G. Holt
University of Westminster
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Jan-1991
Citation: Journal of Biotechnology. Vol.19, No.1 (1991), 117-122
Abstract: Aspergillus oryzae strain U1638 has been transformed to phleomycin resistance using a phleomycin based vector. Protoplasts were generated using Novozyme 234 and regenerated on sucrose-stabilized minimal medium containing 200 μg ml-1phleomycin. A frequency of 3 to 7 transformants per 106viable protoplasts was obtained. All transformants tested showed increased resistance to phleomycin when subcultured onto selective medium and the mitotic stability of the transformants was 100% after growth without selective pressure. The vector sequences appeared to be integrated into the A. oryzae U1638 genome at multiple sites. © 1991.
ISSN: 01681656
Appears in Collections:Scopus 1991-2000

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