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|Title:||Isolation and expression in Escherichia coli of a Xanthomonas oryzae recA-like gene|
Janine E. Trempy
Nicholas P. Ambulos
Chulabhorn Research Institute
Oregon State University
University of Maryland, Baltimore County
|Keywords:||Biochemistry, Genetics and Molecular Biology;Medicine|
|Citation:||Gene. Vol.132, No.1 (1993), 113-118|
|Abstract:||The recA gene from the bacterium Xanthomonas oryzae pv. oryzae (Xoo), a rice pathogen, was cloned based on its ability to complement DNA repair defects of Escherichia coli recA-mutants. The Xoo recA was localized to a 1.3-kb Sau3AI-XhoI fragment and, when cloned into pBR322, specifies increased methylmethanesulfonate and mitomycin C resistance to E. coli recA mutants and allows λ red-gam-to plaque on an E. coli recA-host. An E. coli recA-strain harboring a plasmid containing the Xoo recA-like gene was shown to produce a 40-kDa protein which cross-reacted with an anti-E. coli RecA antibody. A similar molecular mass protein to RecA has been detected in several Xanthomonas pathovars using an anti-E. coli RecA antibody. Furthermore, the cloned Xoo recA was shown to hybridize to genomic DNA from various Xanthomonas pathovars, but not to genomic DNA from other bacteria species under high-stringency hybridization conditions. These results indicate the isolation of the Xoo recA gene. © 1993.|
|Appears in Collections:||Scopus 1991-2000|
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