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Title: Polymerase chain reaction detection of Plasmodium falciparum in mosquitoes
Authors: V. Boonsaeng
S. Panyim
P. Wilairat
A. Tassanakajon
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jan-1993
Citation: Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.87, No.3 (1993), 273-275
Abstract: The polymerase chain reaction (PCR) procedure using a primer set derived from a repetitive deoxyribonucleic acid (DNA) sequence specific to Plasmodium falciparum was used to detect parasite DNA in mosquitoes. In laboratory-infected mosquitoes, PCR could detect as few as 10 sporozoites in a dissected salivary gland and a single oocyst in a dissected midgut. The ability to detect P. falciparum DNA in wild-caught mosquitoes indicated an advantage of the PCR over enzyme-linked immunosorbent assay for the detection of Plasmodium sporozoites in mosquitoes with low-grade parasite infections. © 1993 Oxford University Press.
ISSN: 18783503
Appears in Collections:Scopus 1991-2000

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