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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/22874
Title: Chimeric antibody-binding Vitreoscilla Hemoglobin (VHb) mediates redox-catalysis reaction: New insight into the functional role of VHb
Authors: Yaneenart Suwanwong
Malin Kvist
Chartchalerm Isarankura-Na-Ayudhya
Natta Tansila
Leif Bulow
Virapong Prachayasittikul
Mahidol University
Lunds Universitet
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 22-Aug-2006
Citation: International Journal of Biological Sciences. Vol.2, No.4 (2006), 208-215
Abstract: Experimentation was initiated to explore insight into the redox-catalysis reaction derived from the heme prosthetic group of chimeric Vitreoscilla hemoglobin (VHb). Two chimeric genes encoding chimeric VHbs harboring one and two consecutive sequences of Fc-binding motif (Z-domain) were successfully constructed and expressed in E. coli strain TG1. The chimeric ZVHb and ZZVHb were purified to a high purity of more than 95% using IgG-Sepharose affinity chromatography. From surface plasmon resonance, binding affinity constants of the chimeric ZVHb and ZZVHb to human IgG were 9.7 × 107and 49.1 × 107per molar, respectively. More importantly, the chimeric VHbs exhibited a peroxidase-like activity determined by activity staining on native PAGE and dot blotting. Effects of pH, salt, buffer system, level of peroxidase substrate and chromogen substrate were determined in order to maximize the catalytic reaction. From our findings, the chimeric VHbs displayed their maximum peroxidase-like activity at the neutral pH (∼7.0) in the presence of high concentration (20-40 mM) of hydrogen peroxide. Under such conditions, the detection limit derived from the calibration curve was at 250 ng for the chimeric VHbs, which was approximately 5-fold higher than that of the horseradish peroxidase. These findings reveal the novel functional role of Vitreoscilla hemoglobin indicating a high trend of feasibility for further biotechnological and medical applications. ©2006 Ivyspring International Publisher. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33747846320&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/22874
ISSN: 14492288
Appears in Collections:Scopus 2006-2010

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