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Title: Anti-CHH antibody causes impaired hyperglycemia in Penaeus monodon
Authors: Supattra Treerattrakool
Apinunt Udomkit
Sakol Panyim
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 18-Sep-2006
Citation: Journal of Biochemistry and Molecular Biology. Vol.39, No.4 (2006), 371-376
Abstract: Crustacean hyperglycemic hormone (CHH) plays a major role in controlling glucose level in the haemolymph and also triggers important events during molting and reproductive cycles. In Penaeus monodon, three types of CHH, namely Pem-CHH1, Pem-CHH2 and Pem-CHH3, have been previously characterized. In this study, mouse polyclonal antibody was raised against recombinant Pem-CHH1 that was expressed in Escherichia coli. The anti-Pem-CHH1 antibody recognized all three types of Pem-CHHs but did not cross-react with either related hormone, molt-inhibiting hormone of P. monodon, or unrelated human growth hormone. The hyperglycemic activity in the extract from the eyestalk neural tissues was significantly depleted after incubating with anti-Pem-CHH antibody. Direct injection of the antibody into shrimp caused about 30-50% reduction in the haemolymph glucose level. The result demonstrates the ability of anti-Pem-CHH1 antibody to deplete the activity of CHH in vivo, and thus provides a possibility of using anti-Pem-CHH1 antibody to inhibit the hormone activity as a strategy to modulate growth and reproduction in this species.
ISSN: 12258687
Appears in Collections:Scopus 2006-2010

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