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Title: Unstable lysogeny and pseudolysogeny in Vibrio harveyi siphovirus-like phage 1
Authors: Krit Khemayan
Tirasak Pasharawipas
Orapim Puiprom
Siriporn Sriurairatana
Orasa Suthienkul
Timothy W. Flegel
Mahidol University
Rangsit University
Keywords: Biochemistry, Genetics and Molecular Biology;Environmental Science;Immunology and Microbiology
Issue Date: 1-Feb-2006
Citation: Applied and Environmental Microbiology. Vol.72, No.2 (2006), 1355-1363
Abstract: Exposure of Vibrio harveyi (strain VH1114) to V. harveyi siphovirus-like phage 1 (VHS1) resulted in the production of a low percentage of lysogenized clones of variable stability. These were retrieved most easily as small colonies within dot plaques. Analysis revealed that VHS1 prophage was most likely carried by VH1114 as an episome rather than integrated into the host chromosome. In the late exponential growth phase, lysogenized VH1114 continuously produced VHS1 but also gave rise to a large number of cured progeny. The absence of phage DNA in the cured progeny was confirmed by the absence of VHS1 DNA in Southern blot and PCR assays. Curiously, these very stable, cured subclones did not show the parental phenotype of clear plaques with VHS1 but instead showed turbid plaques, both in overlaid lawns and in dot plaque assays. This phenotypic difference from the original parental isolate suggested that transient lysogeny by VHS1 had resulted in a stable genetic change in the cured clones. Such clones may be called pseudolysogens (i.e., false lysogens), since they have undergone transient lysogeny and have retained some resistance to full lytic phage development, despite the loss of viable or detectable prophage. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
ISSN: 00992240
Appears in Collections:Scopus 2006-2010

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