Simple jQuery Dropdowns
Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/23140
Title: Factorial design applied to a non-aqueous capillary electrophoresis method for the separation of β-agonists
Authors: Oraphan Anurukvorakun
Worapot Suntornsuk
Leena Suntornsuk
Mahidol University
King Mongkuts University of Technology Thonburi
Keywords: Chemistry
Issue Date: 17-Nov-2006
Citation: Journal of Chromatography A. Vol.1134, No.1-2 (2006), 326-332
Abstract: The aim of this work was to study the effects of both chemical and instrumental parameters on the separation of β-agonists (clenbuterol (CLE), salbutamol (SAL) and terbutaline (TER)) by non-aqueous capillary electrophoresis (NACE) method. Due to the number of parameters involved and their interactions, factorial experimental designs (EDs) at two levels was applied to investigate the influence of experimental factors (ionic strength of the background electrolyte (BGE), organic solvent, injection time, voltage and temperature) in sets of several CE responses (resolution, (RS), number of theoretical plate (N), tailing factor (TF) and migration time (tm)). As a compromise between the four responses, the optimum condition was obtained in 18 mM ammonium acetate in methanol (MeOH):acetonitrile (ACN):glacial acetic acid (66:33:1%, v/v/v) using an injection time of 4 s, the voltage and the temperature of 28 kV and 24 °C, respectively. The proposed NACE permitted the baseline separation of the three β-agonists within 10.5 min with good repeatability (%RSD < 3.5%) and linearity (r2> 0.99). The developed method was applicable for the analysis of the β-agonists in syrup and tablets and the NACE condition was compatible with a mass spectrometer detector. © 2006 Elsevier B.V. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33750990469&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/23140
ISSN: 00219673
Appears in Collections:Scopus 2006-2010

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.