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Title: Opisthorchis viverrini: Identification of a glycine-tyrosine rich eggshell protein and its potential as a diagnostic tool for human opisthorchiasis
Authors: Jiraporn Ruangsittichai
Vithoon Viyanant
Suksiri Vichasri-Grams
Prasert Sobhon
Smarn Tesana
Edward Suchart Upatham
Annemarie Hofmann
Günter Korge
Rudi Grams
Mahidol University
Thammasat University
Khon Kaen University
Burapha University
Freie Universitat Berlin
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Nov-2006
Citation: International Journal for Parasitology. Vol.36, No.13 (2006), 1329-1339
Abstract: A cDNA encoding a novel eggshell protein (OvESP) with high-glycine (49.2%) and -tyrosine (27.8%) content was cloned from the human liver fluke Opisthorchis viverrini. In the adult parasite, the RNA products of the OvESP gene are limited to the vitelline follicles. They have a size of 800 nucleotides and are already present in 2-week-old juveniles. Immune sera of hamsters, experimentally infected, and humans, naturally infected with O. viverrini, detect bacterially expressed recombinant OvESP (rOvESP). A rabbit anti-rOvESP antiserum only reacts with the shells of intrauterine eggs in tissue sections of the parasite. Comparison of rOvESP with the parasite's excretion/secretion products as diagnostic tools for human opisthorchiasis shows a higher sensitivity (0.82-0.48) and specificity (0.97-0.91) of the recombinant protein in the ELISA technique. But the observed weak cross-reactivity of immune sera from mice infected with Schistosoma mansoni, Schistosoma mekongi, and Fasciola gigantica in Western blots of rOvESP indicates that the diagnostic quality of this protein might be compromised if infections by other trematodes are present. © 2006 Australian Society for Parasitology Inc.
ISSN: 00207519
Appears in Collections:Scopus 2006-2010

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