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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/23425
Title: Vitrification of mouse oocyte using open pulled straws compared with needles
Authors: Surachai Pornwiroon
Somboon Kunathikom
Orawan Makemaharn
Rattanaphorn Huanaraj
Mahidol University
Keywords: Medicine
Issue Date: 1-Dec-2006
Citation: Journal of the Medical Association of Thailand. Vol.89, No.12 (2006), 2015-2020
Abstract: Objective: To compare the survival rate of mouse oocytes and fertilization rate between using open pulled straws (OPS) and needles for vitrification. Material and Method: Meiosis II oocytes from female C57B/6J mice aged 7-8 weeks were collected and allocated to two groups for vitrification by using OPS or needles. Vitrified oocytes were thawed, morphological survival and fertilization rate were examined. Results: There was no obvious difference between the morphological survival rates of vitrified mouse oocytes using OPS and needles (66.7% vs 64.8%). Proportions Difference 1.9% (95%CI -7.1, 10.7). The vitrified oocytes from the needle had significantly higher percentages of fertilization rate than OPS (76.8% vs 62.5%). Proportions Difference -14.3% (95%CI -24.5, -3.6). Conclusion: Vitrification method of mouse oocytes using needles when compared to OPS provides a similar morphological survival rate and higher fertilization rate.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33845639694&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/23425
ISSN: 01252208
01252208
Appears in Collections:Scopus 2006-2010

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