Simple jQuery Dropdowns
Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/23531
Title: Establishment and characterization of a cholangiocarcinoma cell line (RMCCA-1) from a Thai patient
Authors: Panthip Rattanasinganchan
Kawin Leelawat
Sa Ard Treepongkaruna
Chintana Tocharoentanaphol
Somboon Subwongcharoen
Tuangporn Suthiphongchai
Rutaiwan Tohtong
Mahidol University
Rajavithi Hospital
Chulabhorn Research Institute
Keywords: Medicine
Issue Date: 28-Oct-2006
Citation: World Journal of Gastroenterology. Vol.12, No.40 (2006), 6500-6506
Abstract: Aim: To establish and characterize a new cell line derived from peripheral cholangiocarcinoma of a Thai patient. Methods: The peripheral cholangiocarcinoma specimen surgically obtained from the patient was aseptically processed by washing and mincing before culturing in Ham's F12 medium containing 10% fetal bovine serum. After 3 mo, when the cell line has become homogeneous and stabilized, several features were investigated, including growth characteristics, immunofluorescence staining for cytokeratins, expression of tumor markers, chromosomal analysis by G-banding and multicolour fluorescence in situ hybridization (mFISH), in vitro migration and invasion characteristics. Results: The RMCCA-1 cell line has been established. These cells proliferated as a monolayer with a population doubling time of 48 h. Immunofluorescence staining showed positive staining for human cytokeratin 7 and 19 verifying the biliary epithelial origin. RMCCA-1 secreted carbohydrate antigen 19-9 (CA19-9), but insignificant levels of carcinoembryonic antigen (CEA) and α-fetoprotein (AFP). Chromosome analysis identified aneuploidy karyotypes with a modal chromosome number of 59. RMCCA-1 exhibited a low level of in vitro invasiveness, but a high degree of motility. The cell line exhibited a significant number of chromosomal aberrations as shown by mFISH and G-banding methods. Conclusion: A new cell line derived from peripheral cholangiocarcinoma of a Thai patient has been established. This cell line shows a low level of in vitro invasiveness, but a high degree of motility. It will serve as a valuable tool for further studies on tumor biology, molecular pathogenesis, metastatic mechanism and response to therapeutic drugs of cholangiocarcinoma. © 2006 The WJG Press. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33751010766&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/23531
ISSN: 10079327
Appears in Collections:Scopus 2006-2010

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.