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Title: Detection of heterogeneous, intermediate-vancomycin-resistant Staphylococcus aureus (hVISA) using low-concentration vancomycin disks
Authors: Aroonlug Lulitanond
Aroonwadee Chanawong
Pipat Sribenjalux
Wanlop Kaewkes
Malai Vorachit
Piriyaporn Chongtrakool
Danaisak Leumsai
Pensri Monpou
Khon Kaen University
Mahidol University
Keywords: Medicine
Issue Date: 1-Jul-2006
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.37, No.4 (2006), 761-767
Abstract: Heterogeneous, intermediate-vancomycin-resistant Staphylococcus aureus (hVISA) represents a threat of an incurable infection since the first report in 1997. The method used to detect hVISA isolates is a population analysis profile (PAP); however, it is impractical for routine laboratory analysis. We therefore tested a simple, reliable and inexpensive method for the detection of hVISA. Eighteen isolates of hVISA and 22 of vancomycin-sensitive S. aureus (VSSA) were included. The organisms were tested by the disk diffusion method, using 15-μg vancomycin disks on four different media: Mueller-Hinton agar (MHA), MHA plus 2% NaCl (MHAS), Brain Heart Infusion agar (BHA), and BHA plus 2% NaCl (BHAS). In addition, two different inoculum sizes, bacterial suspensions adjusted to 0.5 and 2.0 McFarland, were tested. The inhibition zone was read independently by three medical technologists after incubation at 37°C for 24 and 48 hours. The use of MHAS with an inoculum size of 2.0 McFarland and 48-hour incubation period yielded the highest sensitivity (94.4%), specificity (81.8%), positive predictive value (80.9%), and negative predictive value (94.7%). The disk diffusion test with 15-μg vancomycin disk is simple and may be used as a screening method for the detection of hVISA.
ISSN: 01251562
Appears in Collections:Scopus 2006-2010

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