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Title: Discriminatory powers of molecular typing techniques for methicillin-resistant Staphylococcus aureus in a University Hospital, Thailand
Authors: Chotechana Wilailuckana
Chanwit Tribuddharat
Chuntima Tiensasitorn
Pintip Pongpech
Penphun Naenna
Siriporn Rugdeekha
Chertsak Dhiraputra
Somwang Danchaivijitr
Khon Kaen University
Mahidol University
Chulalongkorn University
Keywords: Medicine
Issue Date: 1-Mar-2006
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.37, No.2 (2006), 327-334
Abstract: Discriminatory powers of various molecular techniques were evaluated for typing of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Siriraj Hospital, Bangkok, Thailand. Thirty MRSA isolates were randomly selected in this study. They were characterized by pulsed-field gel electrophoresis, ClaI-mecA and ClaI-Tn554 polymorphisms, ribotyping, and PCR-based methods including SCCmec typing, spa and coa gene polymorphism, and repeat units in hypervariable region downstream of mecA. Individual molecular typing technique distinguished those MRSA isolates into 2 to 5 types. Eleven genetic backgrounds of MRSA isolates were elucidated by combination of typing methods with trimethoprim/sulfamethoxazole (TMP/SXT) susceptibility. Combination of all typing methods including TMP/SXT susceptibility yielded a discriminatory index of 0.94. Combination of PCR-based methods and TMP/SXT susceptibility, with the discriminatory index of 0.89, is a practical typing approach suitable for rapid epidemiological investigation of MRSA isolates in a hospital setting.
ISSN: 01251562
Appears in Collections:Scopus 2006-2010

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