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Title: Polymerase chain reaction identification of three members of the Anopheles sundaicus (Diptera: Culicidae) complex, malaria vectors in Southeast Asia
Authors: Isabelle Dusfour
Johanna Blondeau
Ralph E. Harbach
Indra Vythilingham
Visut Baimai
Ho D. Trung
Tho Sochanta
Michael J. Bangs
Sylvie Manguin
IRD Institut de Recherche pour le Developpement
Uniformed Services University of the Health Sciences
The Natural History Museum, London
Institute for Medical Research Kuala Lumpur
Mahidol University
National Institute of Malariology, Parasitology and Entomology Hanoi
National Center for Parasitology, Entomology and Malaria Control
Preventive Medicine Department
Keywords: Agricultural and Biological Sciences;Immunology and Microbiology;Medicine;Veterinary
Issue Date: 1-Sep-2007
Citation: Journal of Medical Entomology. Vol.44, No.5 (2007), 723-731
Abstract: Anopheles sundaicus s.l., a major malaria vector taxon, occurs primarily along coastal areas and on islands in Southeast Asia. Our previous studies using cytochrome oxidase I, cytochrome-b, and internal transcribed spacer 2 markers discriminated three allopatric species: An. sundaicus s.s. in northern Borneo, An. epiroticus in Southeast Asia, and An. sundaicus E on Sumatra and Java, Indonesia. Morphological comparisons of three developmental stages did not reveal unique diagnostic characters that could reliably distinguish the three species. Therefore, we developed a multiplex polymerase chain reaction (PCR) assay based on two mitochondrial DNA markers to unambiguously identify them, This PCR was tested on 374 specimens from 24 different geographical populations, expanding our knowledge of the distribution of these species. © 2007 Entomological Society of America.
ISSN: 00222585
Appears in Collections:Scopus 2006-2010

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