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Title: A pulsed-field gel electrophoresis typing scheme for Vibrio parahaemolyticus isolates from fifteen countries
Authors: Hin chung Wong
Shu Hui Liu
Chien Shun Chiou
Mitsuaki Nishibuchi
Bok Kwon Lee
Orasa Suthienkul
Gopinath Balakrish Nair
Charles A. Kaysner
Hatsumi Taniguchi
Soochow University, Taipei
Center for Disease Control Taiwan
Kyoto University
Korea National Institute of Health
Mahidol University
National Institute of Cholera and Enteric Diseases India
Food and Drug Administration
University of Occupational and Environmental Health
Keywords: Agricultural and Biological Sciences;Immunology and Microbiology
Issue Date: 20-Mar-2007
Citation: International Journal of Food Microbiology. Vol.114, No.3 (2007), 280-287
Abstract: Vibrio parahaemolyticus is an important foodborne pathogen in Taiwan and many other maritime Asian countries where seafood is frequently consumed. A total of 535 strains of V. parahaemolyticus were recovered mostly (97%) from clinical samples obtained in Taiwan or in 14 other countries. These strains were typed by pulsed-field gel electrophoresis following SfiI digestion and a typing scheme was generated. The 115 different patterns identified were grouped into 13 types with dissimilarity values less than 15, plus 16 miscellaneous patterns not grouped into any of the types. Types I, A, D and J contained the most patterns, with the numbers of patterns being 17, 13, 12, and 11, respectively. However, types I, B, D, A, H and C contained the most strains, with the numbers of strains being 204, 73, 71, 54, 29 and 25, respectively. Type I consisted exclusively of the pandemic O3:K6 strains and genetically closely related strains. This PFGE typing scheme for V. parahaemolyticus could be used for the characterization of pathogenic isolates. © 2006 Elsevier B.V. All rights reserved.
ISSN: 01681605
Appears in Collections:Scopus 2006-2010

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