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Title: C-terminal hydrophobic region leads PRSV P3 protein to endoplasmic reticulum
Authors: Sarasate Eiamtanasate
Mila Juricek
Yun Kiam Yap
Mahidol University
Institute of Experimental Botany of the Academy of Sciences of the Czech Republic
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Dec-2007
Citation: Virus Genes. Vol.35, No.3 (2007), 611-617
Abstract: P3 protein is one of the least characterized potyviral proteins in both functions and sub-cellular localization. In this study, we examined the sub-cellular localization of PRSV P3 and its intermediate, P3-6K1by expressing their GFP fusion proteins in onion epidermal cells. Our results showed that both P3- and P3-6K1GFP fusion proteins were localized at the endoplasmic reticulum. Deletion analysis indicated that C-terminal of P3 protein contained localization signal, and a 19 amino acids hydrophobic domain from this region was able to target the GFP fusion protein to endoplasmic reticulum. C-terminal of P3 proteins has been suggested to be involved in both viability and pathogenicity of the potyvirus. Therefore, our result suggests that localization of P3 protein at endoplasmic reticulum is essential for functionality of P3 protein. © 2007 Springer Science+Business Media, LLC.
ISSN: 1572994X
Appears in Collections:Scopus 2006-2010

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