Please use this identifier to cite or link to this item:
|Title:||Analysis of differently expressed proteins and transcripts in gills of Penaeus vannamei after yellow head virus infection|
Hao Ching Wang
Chu Fang Lo
Timothy W. Flegel
National Taiwan University
|Keywords:||Biochemistry, Genetics and Molecular Biology|
|Citation:||Proteomics. Vol.7, No.20 (2007), 3809-3814|
|Abstract:||In this proteomic analysis of gills from yellow head virus (YHV)-infected Penaeus vannamei, we identified 13 spots with up-regulated protein expression levels and five spots with down-regulated levels. LC-nanoESI-MS/MS indicated that the up-regulated proteins included enzymes in the glycolytic pathway, the tricarboxylic acid cycle and amino acid metabolism. The other up-regulated proteins were arginine kinase, imaginal disk growth factor (IDGF) and a Ras-like GTP binding protein. By contrast, expression levels were reduced for an SCP-calcium binding protein (SCP), actin-1, a valosin-containing protein, and Rab11. Time-course assays by real time RT-PCR revealed no significant increase in mRNA level of glycolytic enzymes and arginine kinase. However, a significant decrease in SCP mRNA was observed. The present results are consistent with previously published work and suggest that a decrease in SCP expression may play an important role in the shrimp response to viral infections in general. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.|
|Appears in Collections:||Scopus 2006-2010|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.