Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/24108
Title: Analysis of differently expressed proteins and transcripts in gills of Penaeus vannamei after yellow head virus infection
Authors: Triwit Rattanarojpong
Hao Ching Wang
Chu Fang Lo
Timothy W. Flegel
Mahidol University
National Taiwan University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Oct-2007
Citation: Proteomics. Vol.7, No.20 (2007), 3809-3814
Abstract: In this proteomic analysis of gills from yellow head virus (YHV)-infected Penaeus vannamei, we identified 13 spots with up-regulated protein expression levels and five spots with down-regulated levels. LC-nanoESI-MS/MS indicated that the up-regulated proteins included enzymes in the glycolytic pathway, the tricarboxylic acid cycle and amino acid metabolism. The other up-regulated proteins were arginine kinase, imaginal disk growth factor (IDGF) and a Ras-like GTP binding protein. By contrast, expression levels were reduced for an SCP-calcium binding protein (SCP), actin-1, a valosin-containing protein, and Rab11. Time-course assays by real time RT-PCR revealed no significant increase in mRNA level of glycolytic enzymes and arginine kinase. However, a significant decrease in SCP mRNA was observed. The present results are consistent with previously published work and suggest that a decrease in SCP expression may play an important role in the shrimp response to viral infections in general. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=35648986063&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/24108
ISSN: 16159861
16159853
Appears in Collections:Scopus 2006-2010

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.