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Title: | Analysis of differently expressed proteins and transcripts in gills of Penaeus vannamei after yellow head virus infection |
Authors: | Triwit Rattanarojpong Hao Ching Wang Chu Fang Lo Timothy W. Flegel Mahidol University National Taiwan University |
Keywords: | Biochemistry, Genetics and Molecular Biology |
Issue Date: | 1-Oct-2007 |
Citation: | Proteomics. Vol.7, No.20 (2007), 3809-3814 |
Abstract: | In this proteomic analysis of gills from yellow head virus (YHV)-infected Penaeus vannamei, we identified 13 spots with up-regulated protein expression levels and five spots with down-regulated levels. LC-nanoESI-MS/MS indicated that the up-regulated proteins included enzymes in the glycolytic pathway, the tricarboxylic acid cycle and amino acid metabolism. The other up-regulated proteins were arginine kinase, imaginal disk growth factor (IDGF) and a Ras-like GTP binding protein. By contrast, expression levels were reduced for an SCP-calcium binding protein (SCP), actin-1, a valosin-containing protein, and Rab11. Time-course assays by real time RT-PCR revealed no significant increase in mRNA level of glycolytic enzymes and arginine kinase. However, a significant decrease in SCP mRNA was observed. The present results are consistent with previously published work and suggest that a decrease in SCP expression may play an important role in the shrimp response to viral infections in general. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA. |
URI: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=35648986063&origin=inward http://repository.li.mahidol.ac.th/dspace/handle/123456789/24108 |
ISSN: | 16159861 16159853 |
Appears in Collections: | Scopus 2006-2010 |
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