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dc.contributor.authorHathaitip Sritanaudomchaien_US
dc.contributor.authorKanok Pavasuthipaisiten_US
dc.contributor.authorYindee Kitiyananten_US
dc.contributor.authorPiengchai Kupradinunen_US
dc.contributor.authorShoukhrat Mitalipoven_US
dc.contributor.authorThanit Kusamranen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherThe Institute of Science and Technology for Research and Development, Mahidol Universityen_US
dc.contributor.otherNational Cancer Institute Thailanden_US
dc.contributor.otherOregon National Primate Research Centeren_US
dc.date.accessioned2018-08-24T01:40:07Z-
dc.date.available2018-08-24T01:40:07Z-
dc.date.issued2007-10-01en_US
dc.identifier.citationMolecular Reproduction and Development. Vol.74, No.10 (2007), 1295-1302en_US
dc.identifier.issn10982795en_US
dc.identifier.issn1040452Xen_US
dc.identifier.other2-s2.0-35848947712en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=35848947712&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/24112-
dc.description.abstractEmbryonic stem (ES) cells derived from mammalian embryos have the ability to form any terminally differentiated cell of the body. We herein describe production of parthenogenetic buffalo (Bubalus Bubalis) blastocysts and subsequent isolation of an ES cell line. Established parthenogenetic ES (PGES) cells exhibited diploid karyotype and high telomerase activity. PGES cells showed remarkable long-term proliferative capacity providing the possibility for unlimited expansion in culture. Furthermore, these cells expressed key ES cell-specific markers defined for primate species including stage-specific embryonic antigen-4 (SSEA-4), tumor rejection antigen-1-81 (TRA-1-81), and octamer-binding transcription factor 4 (Oct-4). In vitro, in the absence of a feeder layer, cells readily formed embryoid bodies (EBs). When cultured for an extended period of time, EBs spontaneously differentiated into derivatives of three embryonic germ layers as detected by PCR for ectodermal (nestin, oligodendrocytes, and tubulin), mesodermal (scleraxis, α-skeletal actin, collagen II, and osteocalcin) and endodermal markers (insulin and α-fetoprotein). Differentiation of PGES cells toward chondrocyte lineage was directed by supplementing serum-containing media with ascorbic acid, β-glycerophosphate, and dexamethasone. Moreover, when PGES cells were injected into nude mice, teratomas with derivatives representing all three embryonic germ layers were produced. Our results suggest that the cell line isolated from a parthenogenetic blastocyst holds properties of ES cells, and can be used as an in vitro model to study the effects of imprinting on cell differentiation and as an a invaluable material for extensive molecular studies on imprinted genes. © 2007 Wiley-Liss, Inc.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=35848947712&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleCharacterization and multilineage differentiation of embryonic stem cells derived from a buffalo parthenogenetic embryoen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1002/mrd.20592en_US
Appears in Collections:Scopus 2006-2010

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