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|Title:||pH-imprinted lipase catalyzed synthesis of dextran fatty acid ester|
Laboratoire de Chimie Physique Macromoleculaire
|Keywords:||Biochemistry, Genetics and Molecular Biology;Chemical Engineering|
|Citation:||Journal of Molecular Catalysis B: Enzymatic. Vol.47, No.3-4 (2007), 135-142|
|Abstract:||The application of enzymatic catalysis for the synthesis of polysaccharide-based surfactants was investigated. The polysaccharide dextran, a neutral bacterial polysaccharide consisting of α-1,6 linked glucose units, was chemically modified by the attachment of hydrophobic groups through a transesterification reaction with a vinyl decanoate. A screening of commercially available lipases and protease for the synthesis of amphiphilic polysaccharides in DMSO suggested that lipase AY from Candida rugosa modified dextran T-40 with vinyl decanoate at the highest conversion. A pH-adjustment in a phosphate buffer at pH 7.5 prior to use is crucial to make this enzyme active in DMSO. The effect of enzyme concentration and mole ratio of fatty ester to dextran T-40 on the conversion and the rate of reaction were studied. Finally, investigation of the kinetics and regioselectivity of lipase AY-catalyzed modification offer a possibility to regulate the position and the extent of hydrophobic group attached to dextran. These two properties are fundamental for controlling the physico-chemical properties of the final polymeric surfactants. © 2007 Elsevier B.V. All rights reserved.|
|Appears in Collections:||Scopus 2006-2010|
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