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dc.contributor.authorPiyada Wangroongsarben_US
dc.contributor.authorTeerarut Chanketen_US
dc.contributor.authorKata Gunlabunen_US
dc.contributor.authorDo Hoang Longen_US
dc.contributor.authorPedcharat Satheanmethakulen_US
dc.contributor.authorSiriporn Jetanadeeen_US
dc.contributor.authorJanjira Thaipadungpaniten_US
dc.contributor.authorVannaporn Wuthiekanunen_US
dc.contributor.authorSharon J. Peacocken_US
dc.contributor.authorStuart D. Blacksellen_US
dc.contributor.authorLee D. Smytheen_US
dc.contributor.authorDieter M. Bulachen_US
dc.contributor.authorThareerat Kalambahetien_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherWorld Health Organization, Australiaen_US
dc.contributor.otherMonash Universityen_US
dc.identifier.citationFEMS Microbiology Letters. Vol.271, No.2 (2007), 170-179en_US
dc.description.abstractMolecular typing of leptospiral strains based on variation within putative O-antigen polymerase gene (wzy) was determined among reference strains and those isolated from patients. Using the PCR primers designed from the flanking gene of wzy derived from Leptospira interrogans serovar Copenhageni, all L. interrogans serovars as well as human and rodent leptospiral isolates from Thailand could be amplified. The size of PCR product ranged from 1 to 1.5 kb. The limitation of these primer pairs was the inability to amplify those strains whose sequences differ in the region of the primers, these included Leptospira biflexa (serovar Patoc), Leptospira borgpetersenii (serovar Tarassovi) and Leptospira kirschneri (serovar Bim, Bulgarica, Butembo). Notably, amplification was not limited to L. interrogans as demonstrated by the amplification of some strains from L. kirschneri, Leptospira meyeri, Leptospira noguchii, Leptospira santarosai, L. borgpetersenii and Leptospira weilii. The phylogenetic tree of wzy sequence, inferred by posterior probability of the Bayesian, enabled the categorization of leptospiral serovars into seven genetically related group, of which its differentiation power was better than that of the more highly conserved 16S rRNA gene, which is used extensively for genotyping. © 2007 Federation of European Microbiological Societies.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleMolecular typing of Leptospira spp. based on putative O-antigen polymerase gene (wzy), the benefit over 16S rRNA gene sequenceen_US
Appears in Collections:Scopus 2006-2010

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