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Title: Induction of RNA-mediated resistance to papaya ringspot virus type W
Authors: Pongrit Krubphachaya
Míla Juříček
Sunee Kertbundit
Mahidol University
Institute of Experimental Botany of the Academy of Sciences of the Czech Republic
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-May-2007
Citation: Journal of Biochemistry and Molecular Biology. Vol.40, No.3 (2007), 404-411
Abstract: Transformation of cantaloupes with the coat protein (cp) gene of papaya ringspot virus type W (PRSV-W), Thai isolate, was used to introduce virus resistance. Binary vectors containing either the full length coat protein coding region under control of the 35S CaMV promoter (pSA1175), or the inverted-repeat of a coat protein coding region (pSA1304), were constructed and used for Agrobacterium-mediated transformation of cotyledonary explants of the cantaloupe cultivar Sun Lady. Four independent transgenic lines were obtained using pSA1304 and one using pSA1175. Integration of the PRSV-W cp gene into the genome of these transgenic lines was verified by PCR amplification, GUS assays and Southern blot hybridization. In vitro inoculation of these lines with PRSV-W revealed that whereas the line containing pSA1175 remained sensitive, the four lines containing pSA1304 were resistant. The presence of small RNA species, presumably siRNA, corresponding to regions of the viral cp gene in transgenic lines resistant to PRSV-W supports the involvement of post-transcriptional gene silencing in the establishment of resistance.
ISSN: 02191024
Appears in Collections:Scopus 2006-2010

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