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Title: Protection of cells from oxidative stress by microsomal glutathione transferase 1
Authors: Atchasai Siritantikorn
Katarina Johansson
Karin Åhlen
Rosanna Rinaldi
Tuangporn Suthiphongchai
Prapon Wilairat
Ralf Morgenstern
Mahidol University
Chulalongkorn University
Karolinska Institutet
IRCCS San Raffaele Scientific Institute
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 6-Apr-2007
Citation: Biochemical and Biophysical Research Communications. Vol.355, No.2 (2007), 592-596
Abstract: Rat liver microsomal glutathione transferase 1 (MGST1) is a membrane-bound enzyme that displays both glutathione transferase and glutathione peroxidase activities. We hypothesized that physiologically relevant levels of MGST1 is able to protect cells from oxidative damage by lowering intracellular hydroperoxide levels. Such a role of MGST1 was studied in human MCF7 cell line transfected with rat liver mgst1 (sense cell) and with antisense mgst1 (antisense cell). Cytotoxicities of two hydroperoxides (cumene hydroperoxide (CuOOH) and hydrogen peroxide) were determined in both cell types using short-term and long-term cytotoxicity assays. MGST1 significantly protected against CuOOH and against hydrogen peroxide (although less pronounced and only in short-term tests). These results demonstrate that MGST1 can protect cells from both lipophilic and hydrophilic hydroperoxides, of which only the former is a substrate. After CuOOH exposure MGST1 significantly lowered intracellular ROS as determined by FACS analysis. © 2007 Elsevier Inc. All rights reserved.
ISSN: 10902104
Appears in Collections:Scopus 2006-2010

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