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dc.contributor.authorAmornrat Aroonnualen_US
dc.contributor.authorTakuya Nihiraen_US
dc.contributor.authorTatsuji Sekien_US
dc.contributor.authorWatanalai Panbangreden_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherOsaka Universityen_US
dc.date.accessioned2018-08-24T01:42:21Z-
dc.date.available2018-08-24T01:42:21Z-
dc.date.issued2007-04-03en_US
dc.identifier.citationEnzyme and Microbial Technology. Vol.40, No.5 (2007), 1221-1227en_US
dc.identifier.issn01410229en_US
dc.identifier.other2-s2.0-33847758653en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33847758653&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/24212-
dc.description.abstractThe gene encoding sucrose isomerase (palI NK33) was cloned from Klebsiella pneumoniae strain NK33-98-8. The gene was over-expressed in Escherichia coli BL21 (DE3) pLysS and its enzyme product (PalI NK33) was purified and characterized. PalI NK33 converts sucrose to 76.8% palatinose, 21.2% trehalulose and 1% each of glucose and fructose. The purified PalI NK33 showed the very high specific activity at 2362 U/mg and Kmfor sucrose was 42.7 ± 0.75 mM (at pH 6.0 and 30 °C). The enzyme activity was completely inhibited by 1 mM concentration of either Hg2+or SDS. Ca2+, Li2+and Mg2+at 1 mM slightly enhanced enzyme activity. Mutations on Asp140, located within the conserved sequence region I to either glutamic acid, glycine or asparagine had drastically reduced enzyme activity. The change of amino acid residues in the sequence325RLDRD329to325RYDRA329reduced enzyme activity 24-fold and did not affect ratio of palatinose and trehalulose formation. © 2006 Elsevier Inc. All rights reserved.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33847758653&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleRole of several key residues in the catalytic activity of sucrose isomerase from Klebsiella pneumoniae NK33-98-8en_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1016/j.enzmictec.2006.09.011en_US
Appears in Collections:Scopus 2006-2010

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