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dc.contributor.authorVisith Thongboonkerden_US
dc.contributor.authorMuthita Vanapornen_US
dc.contributor.authorNapat Songtaweeen_US
dc.contributor.authorRattiyaporn Kanlayaen_US
dc.contributor.authorSupachok Sinchaikulen_US
dc.contributor.authorShui Tein Chenen_US
dc.contributor.authorAnna Eastonen_US
dc.contributor.authorKaren Chuen_US
dc.contributor.authorGregory J. Bancroften_US
dc.contributor.authorSunee Korbsrisateen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherAcademia Sinica Taiwanen_US
dc.contributor.otherNational Taiwan Universityen_US
dc.contributor.otherLondon School of Hygiene & Tropical Medicineen_US
dc.contributor.otherFaculty of Medicine, Siriraj Hospital, Mahidol Universityen_US
dc.date.accessioned2018-08-24T01:42:39Z-
dc.date.available2018-08-24T01:42:39Z-
dc.date.issued2007-04-01en_US
dc.identifier.citationJournal of Proteome Research. Vol.6, No.4 (2007), 1334-1341en_US
dc.identifier.issn15353893en_US
dc.identifier.other2-s2.0-34248169257en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34248169257&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/24224-
dc.description.abstractWe have previously shown that the alternative sigma factor σE (RpoE), encoded by rpoE, is involved in stress tolerance and survival of Burkholderia pseudomallei. However, its molecular and pathogenic mechanisms remain unclear. In the present study, we applied gel-based, differential proteomics to compare the cellular proteome of an rpoE operon knockout mutant (RpoE Mut) to that of wild-type (K96243 WT) B. pseudomallei. Quantitative intensity analysis (n = 5 gels from 5 individual culture flasks in each group) revealed significantly differential expression of 52 proteins, which were subsequently identified by Q-TOF MS/MS. These included oxidative, osmotic, and other stress response proteins; chaperones; transcriptional/translational regulators; metabolic enzymes; proteins involved in cell wall synthesis, fatty synthesis, glycogen synthesis, and storage; exported proteins; secreted proteins; adhesion molecule; protease/peptidase; protease inhibitor; signaling proteins; and other miscellaneous proteins. The down-regulation of several stress response proteins, chaperones, transcriptional/translational regulators, and proteins involved in cell wall synthesis in RpoE Mut provided some new insights into the mechanisms of the rpoE operon for the stress tolerance and survival of B. pseudomallei. In addition, the proteomic data and in vivo study indicated that the rpoE operon is also involved in the virulence of B. pseudomallei. Our findings underscore the usefulness of proteomics for unraveling pathogenic mechanisms of diseases at the molecular level. © 2007 American Chemical Society.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34248169257&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleAltered proteome in Burkholderia pseudomallei rpoE operon knockout mutant: Insights into mechanisms of rpoE operon in stress tolerance, survival, and virulenceen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1021/pr060457ten_US
Appears in Collections:Scopus 2006-2010

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