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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/24229
Title: Genomic analysis of the Mozambique strain of Vibrio cholerae O1 reveals the origin of El Tor strains carrying classical CTX prophage
Authors: Shah M. Faruque
Vincent C. Tam
Nityananda Chowdhury
Pornphan Diraphat
Michelle Dziejman
John F. Heidelberg
John D. Clemens
John J. Mekalanos
G. Balakrish Nair
International Centre for Diarrhoeal Disease Research Bangladesh
Mahidol University
University of Rochester School of Medicine and Dentistry
J. Craig Venter Institute
International Vaccine Institute, Seoul
Harvard Medical School
Keywords: Biochemistry, Genetics and Molecular Biology;Multidisciplinary
Issue Date: 20-Mar-2007
Citation: Proceedings of the National Academy of Sciences of the United States of America. Vol.104, No.12 (2007), 5151-5156
Abstract: Cholera outbreaks in subSaharan African countries are caused by strains of the El Tor biotype of toxigenic Vibrio cholerae O1. The El Tor biotype is the causative agent of the current seventh cholera pandemic, whereas the classical biotype, which was associated with the sixth pandemic, is now extinct. Besides other genetic differences the CTX prophages encoding cholera toxin in the two biotypes of V. cholerae O1 have distinct repressor (rstR) genes. However, recent incidences of cholera in Mozambique were caused by an El Tor biotype V. cholerae O1 strain that, unusually, carries a classical type (CTX class) prophage. We conducted genomic analysis of the Mozambique strain and its CTX prophage together with chromosomal phage integration sites to understand the origin of this atypical strain and its evolutionary relationship with the true seventh pandemic strain. These analyses showed that the Mozambique strain carries two copies of CTXclass prophage located on the small chromosome in a tandem array that allows excision of the prophage, but the excised phage genome was deficient in replication and did not produce CTXclass virion. Comparative genomic microarray analysis revealed that the strain shares most of its genes with the typical El Tor strain N16961 but did not carry the TLC gene cluster, and RS1 sequence, adjacent to the CTX prophage. Our data are consistent with the Mozambique strain's having evolved from a progenitor similar to the seventh pandemic strain, involving multiple recombination events and suggest a model for origination of El Tor strains carrying the classical CTX prophage. © 2007 by The National Academy of Sciences of the USA.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34247587641&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/24229
ISSN: 00278424
Appears in Collections:Scopus 2006-2010

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