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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/24245
Title: Control of ion transport in mouse proximal and distal colon by prolactin
Authors: Supaporn Puntheeranurak
Rainer Schreiber
Melanie Spitzner
Jiraporn Ousingsawat
Nateetip Krishnamra
Karl Kunzelmann
Universitat Regensburg
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 23-Feb-2007
Citation: Cellular Physiology and Biochemistry. Vol.19, No.1-4 (2007), 77-88
Abstract: The lactogenic hormone prolactin (PRL) has been known to affect Ca 2+ and electrolyte transport in the intestinal epithelium. In the present study we analyzed ion transport in mouse proximal and distal colon, and acute changes induced by PRL. In the proximal colon, carbachol activated a Ca2+ dependent Cl- secretion that was sensitive to DIDS and NFA. In the distal colon, both ATP and carbachol activated K+ secretion. Ca2+ -activated KCl transport in proximal and distal colon was inhibited by PRL (200ng/ml), while amiloride sensitive Na+ absorption and cAMP induced Cl- secretion remained unaffected. Luminal large conductance Ca2+ -activated K+ (BK) channels were largely responsible for Ca2+ -activated K+ secretion in the distal colon, and basolateral BK channels supported Ca2+ -activated Cl- secretion in the proximal colon. Ca2+ chelating by BAPTA-AM attenuated effects of carbachol and abolished effects of PRL. Both inhibition of PI3 kinase with wortmannin and blockage of MAP kinases with SB 203580 or U 0126, interfered with the acute inhibitory effect of PRL on ion transport, while blocking of Jak/Stat kinases with AG 490 was without effects. PRL attenuated the increase in intracellular Ca2+ that was caused by stimulation of isolated colonic crypts with carbachol. Thus PRL inhibits Ca2+ dependent Cl- and K+ secretion by interfering with intracellular Ca2+ signaling and probably by activating PI3 kinase and MAP kinase pathways. Copyright © 2007 S. Karger AG.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33847055913&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/24245
ISSN: 10158987
Appears in Collections:Scopus 2006-2010

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