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Title: Proteomics of human urine
Authors: Visith Thongboonkerd
Pedro R. Cutillas
Robert J. Unwin
Stefan Schaub
Peter Nickerson
Marion Haubitz
Harald Mischak
Dobrin Nedelkov
Urban A. Kiernan
Randall W. Nelson
Mahidol University
UCL Medical School
Manitoba Centre for Proteomics
Universitatsspital Basel
University of Manitoba
Medizinische Hochschule Hannover (MHH)
Mosaiques Diagnostics and Therapeutics AG
Intrinsic Bioprobes Incorporated
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Jan-2007
Citation: Proteomics of Human Body Fluids: Principles, Methods, and Applications. (2007), 225-268
Abstract: © 2007 Humana Press Inc. All rights reserved. Urine is one of the most interesting and useful human body fluids for clinical proteomics studies because of its availability in almost all patients and ease of collection without any invasive or painful procedure. Proteomic analysis of human urine offers invaluable information not only for a better understanding of the normal renal physiology and pathophysiology of kidney/kidney-related diseases, but also for biomarker discovery. This chapter provides principles, brief methods, and applications of various proteomic technologies (i.e., gel-based method, liquid chromatographybased approach, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, capillary electrophoresis coupled to mass spectrometry, and mass spectrometric immunoassay) to examine the human urine. At the end, perspectives and future directions in the field of urinary proteomics are discussed.
Appears in Collections:Scopus 2006-2010

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