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Title: Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis
Authors: Narisara Chantratita
Vanaporn Wuthiekanun
Aunchalee Thanwisai
Direk Limmathurotsakul
Allen C. Cheng
Wirongrong Chierakul
Nicholas P.J. Day
Sharon J. Peacock
Mahidol University
Menzies School of Health Research
Nuffield Department of Clinical Medicine
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology;Medicine
Issue Date: 1-Jan-2007
Citation: Clinical and Vaccine Immunology. Vol.14, No.1 (2007), 110-113
Abstract: Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%). Copyright © 2007, American Society for Microbiology. All Rights Reserved.
ISSN: 1556679X
Appears in Collections:Scopus 2006-2010

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