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Title: Development and validation of an automated solid-phase extraction and liquid chromatographic method for determination of lumefantrine in capillary blood on sampling paper
Authors: D. Blessborn
S. Römsing
A. Annerberg
D. Sundquist
A. Björkman
N. Lindegardh
Y. Bergqvist
Hogskolan Dalarna
Uppsala Universitet
Center for Clinical Research Dalarna
Mahidol University
Nuffield Department of Clinical Medicine
Karolinska Institutet
Keywords: Chemistry;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 18-Oct-2007
Citation: Journal of Pharmaceutical and Biomedical Analysis. Vol.45, No.2 (2007), 282-287
Abstract: A bioanalytical method for the determination of lumefantrine in 100 μl blood applied onto sampling paper, by solid-phase extraction and liquid chromatography, has been developed and validated. Whatman 31 ET Chr sampling paper was pre-treated with 0.75 M tartaric acid before sampling capillary blood to enable a high recovery of lumefantrine. Lumefantrine was extracted from the sampling paper, then further purified using solid-phase extraction and finally quantified with HPLC. The between-day variation was below 10% over the range 0.4-25 μM. The lower limit of quantification was 0.25 μM in 100 μl capillary blood. No decrease in lumefantrine concentration in dried blood spot is seen after 4 months storage at 22 °C. The method was also evaluated in field samples from patients in Tanzania after treatment with lumefantrine/artemether. Lumefantrine could be estimated accurately enough to assess bioavailability and treatment compliance on day 7 (i.e. 4 days after the last dose) after a standard regimen with the lumefantrine/artemether combination. © 2007 Elsevier B.V. All rights reserved.
ISSN: 07317085
Appears in Collections:Scopus 2006-2010

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