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Title: ELISA as an alternative tool for epidemiological surveillance for dengue in mosquitoes: A report from Thailand
Authors: Mayuna Srisuphanunt
Ratana Sithiprasasna
Somboon Patpoparn
Watcharee Attatippaholkun
Viroj Wiwanitkit
Mahidol University
Armed Forces Research Institute of Medical Sciences, Thailand
Thailand Ministry of Public Health
Chulalongkorn University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Dec-2007
Citation: Journal of Vector Borne Diseases. Vol.44, No.4 (2007), 272-276
Abstract: Background & objectives: Dengue fever (DF), dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) are the re-emerging infectious diseases caused by the four serotypes of dengue (DEN) virus, type 1 to 4, belonging to the family Flaviviridae and genus Flavivirus. In the absence of a safe and effective mass immunisation, the prevention and control of dengue outbreaks depend upon the surveillance of cases and mosquito vector. The aim of this work is to test enzyme-linked immunosorbent assay (ELISA) tool for the virological surveillance of dengue. Methods: Virus-infected Aedes mosquitoes were collected from the field in order to serve as an early warning monitoring tool for dengue outbreaks. In a prospective field study conducted from April to September 2000, female adult Aedes mosquitoes were caught from selected dengue-sensitive area in Chombung district, Ratchaburi province and assayed by ELISA. Result: Approximately 18.3% were found positive for dengue virus. Conclusion: This can imply that ELISA can be an alternative tool for epidemiological surveillance for dengue in mosquitoes.
ISSN: 09729062
Appears in Collections:Scopus 2006-2010

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