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Title: In vitro antimalarial drug susceptibility and pfcrt mutation among fresh Plasmodium falciparum isolates from the Lao PDR (Laos)
Authors: Mayfong Mayxay
Marion Barends
Alan Brockman
Anchalee Jaidee
Shalini Nair
Dan Sudimack
Tiengkham Pongvongsa
Samlane Phompida
Rattanaxay Phetsouvanh
Tim Anderson
Nicholas J. White
Paul N. Newton
Mahosot Hospital
National University of Laos
Shoklo Malaria Research Unit
Churchill Hospital
Mahidol University
University of Queensland
Texas Biomedical Research Institute
Savannakhet Provincial Malaria Station
Centre of Malariology
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Feb-2007
Citation: American Journal of Tropical Medicine and Hygiene. Vol.76, No.2 (2007), 245-250
Abstract: Recent drug trials in Laos have shown high levels of Plasmodium falciparum resistance to chloroquine, but there are no published data on in vitro antimalarial drug susceptibility. We used the double-site enzyme-linked pLDH immunodetection (DELI) assay to estimate the in vitro antimalarial drug susceptibility of 108 fresh P. falciparum isolates from southern Laos. The geometric mean (95% confidence interval) 50% inhibitory concentration values (nmol/L) were 152.4 (123.8-187.6) for chloroquine, 679.8 (533.8-863.0) for quinine, 45.9 (37.9-55.7) for mefloquine, 5.0 (4.4-6.4) for artesunate, 6.3 (4.5-8.9) for dihydroartemisinin, and 59.1 (46.4-75.3) for lumefantrine. The proportion of isolates defined as resistant were 65%, 40%, and 8% for chloroquine, quinine, and mefloquine, respectively. Of 53 isolates genotyped for the pfcrt T76K chloroquine-resistance mutation, 48 (91%) were mutants. P. falciparum in Laos is multi-drug resistant; antimalarial immunity resulting from the use of ineffective chloroquine before 2005 probably contributes significantly to the therapeutic responses in clinical trials. Copyright © 2007 by The American Society of Tropical Medicine and Hygiene.
ISSN: 00029637
Appears in Collections:Scopus 2006-2010

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