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Title: Biochemical characterisation of two forms of halo- and thermo-tolerant chitinase C of Salinivibrio costicola expressed in Escherichia coli
Authors: Ratchaneewan Aunpad
David W. Rice
Svetalana Sedelnikova
Watanalai Panbangred
Thammasat University
University of Sheffield
Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 1-Jan-2007
Citation: Annals of Microbiology. Vol.57, No.2 (2007), 249-257
Abstract: Two forms of chitinase C (Chi-I and Chi-II) were purified until homogeneity from the culture supernatant of a transformant Escherichia coli harbouring chitinase C gene from the halophilic bacterium Salinivibrio costicola strain 5SM-1. Chi-II was derived from Chi-I by C-terminal processing. Chi-I and Chi-II showed similar salinity optimum at 1-2% NaCl and retained more than 80% of their activity at 3-5% NaCl and more than 50% residual activity at 14% NaCI. The two enzymes could also well function (activity > 95%) in the absence of NaCI. Both had highest activity at pH 7.0 and 50°C and both were stable over a wide range of pH (3.0-10.0). More than 50% activity remained at 80°C after 60 min treatment. Among 4 major cations contained in sea water, only Mg2+at 10 mM increased activity about 10%. Using ρ-nitrophenyl-N, N′-diacetylchitobiose as substrate, Chi-I and Chi-II had Kmof 30 and 31.8 μM and Vmaxof 10 and 9.2 pmol/h/mg protein, respectively. Chi-I and Chi-II were classified as exochitinases by product analysis of the E. coli culture supernatant with high performance liquid chromatography (HPLC) and thin-layer chromatography (TLC).
ISSN: 15904261
Appears in Collections:Scopus 2006-2010

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