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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/24648
Title: Cloning, protein expression and immunogenicity of HBs-murine IL-18 fusion DNA vaccine
Authors: Sunee Channarong
Ampol Mitrevej
Nuttanan Sinchaipanid
Kanchana Usuwantim
Kasem Kulkeaw
Wanpen Chaicumpa
Mahidol University
Thammasat University
Keywords: Medicine
Issue Date: 1-Dec-2007
Citation: Asian Pacific Journal of Allergy and Immunology. Vol.25, No.4 (2007), 233-242
Abstract: Hepatitis B is a global serious disease caused by hepatitis B virus (HBV). There is no known cure for hepatitis B. The best way to deal with the disease is by preventing with hepatitis B vaccine. However, the current protein-based vaccines made up of recombinant hepatitis B surface antigen (HBsAg) are ineffective in chronic HBV carriers and a significant number of the vaccinees do not mount the protective immune response. Novel DNA-based immunization may overcome the deficits of the protein-based immunization and may provide more effective prophylactic and therapeutic outcomes. In this study, we constructed a recombinant plasmid carrying gene encoding the HBV surface antigen (HBs) linked to DNA segment encoding full-length murine interleukin-18, i.e. pcDNA-HBs-L-18. Immunogenicity of the DNA construct was carried out in BALB/c mice in comparison with mock, i.e. pcDNA3.1+ and vaccines comprised of pRc/CMV-HBs and pRc/CMV-HBs plus pcDNA-IL-18. All vaccinated mice revealed significant serum anti-HBs IgG response after two intramuscular in actions of the vaccines at 28 day interval as compared to the level of mock. Co-administration of pRc/CMV-HBs and pcDNA-IL-18 elicited arbitrarily higher levels of anti-HBs IgG than the levels in mice immunized with pRc/CMV-HBs alone and mice that received pcDNA-HBs-IL-18 although not statistically different. Further experiments are needed to investigate the sub-isotypes of the IgG antibody, the kinetics of cytokine and the cell-mediated immune response. For this communication, the prototype HBs-IL-18 DNA vaccine was successfully constructed and the gene encoding murine IL-18 was successfully cloned. The latter can be co-injected with the antigen coding DNA or used as a fusion partner to the DNA for priming the immune response. The recombinant HBs and full-length IL-18 proteins have potential for other research purposes. They may be used also as standard proteins in the protein quantification assay.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=40749141637&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/24648
ISSN: 0125877X
Appears in Collections:Scopus 2006-2010

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