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Title: Sensitive detection of Pneumocystis jirovecii DNA in gastric wash using nested polymerase chain reaction
Authors: Suvit Limawongpranee
Darawan Wanachiwanawin
Kulkanya Chokephaibulkit
Jerawan Onchrotchanakun
Punpop Lertlaituan
Suphaluck Wankhom
Mahidol University
Keywords: Medicine
Issue Date: 1-Sep-2007
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.38, No.5 (2007), 892-896
Abstract: One hundred and five samples of gastric washes were obtained from 52 pediatric patients. Eleven of the 105 samples (10%) gave positive results using immunofluorescence antibody test (IFA) for Pneumocystis jirovecii. Single-step polymerase chain reaction (PCR) produced 13 % (14 samples), whereas detection by nested PCR was increased to 65 samples (62%). Moderate agreement (kappa = 0.5) was found between test results of IFA and single-step PCR, but no agreement was found between the results of IFA and nested PCR (kappa = 0.1).
ISSN: 01251562
Appears in Collections:Scopus 2006-2010

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