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Title: Patient and sample-related factors that effect the success of in vitro isolation of Orientia tsutsugamushi
Authors: Rungnapa Luksameetanasan
Stuart D. Blacksell
Thareerat Kalambaheti
Vanaporn Wuthiekanun
Wirongrong Chierakul
Sunee Chueasuwanchai
Apichat Apiwattanaporn
John Stenos
Stephen Graves
Sharon J. Peacock
N. P J Day
Mahidol University
John Radcliffe Hospital
Udon Thani Center Hospital
Geelong Hospital
Keywords: Medicine
Issue Date: 1-Jan-2007
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.38, No.1 (2007), 91-96
Abstract: Orientia tsutsugamushi is the causative agent of scrub typhus infection, a major cause of human disease in rural areas of Southeast Asia. Twenty-six blood samples collected from patients with serologically proven scrub typhus during a six month period were sent to Bangkok (535 km from the clinical site) by road at ambient temperature (average daily temperature range: 27.1-29.1°C) for attempted in vitro isolation in Vero cells. O. tsutsugamushi was isolated from 12 samples (sensitivity 46.7%) with the time to isolation ranging from 16 to 37 days [median 27 days, inter-quartile range (IQR) 22.5-33.5 days]. Patient factors such as days of fever and O. tsutsugamushi IgM antibody titer, transport factors such as transit time, and isolate genotype (Karp and Gilliam/Kawasaki) were assessed to determine their influence on the outcome of in vitro isolation. None of the factors significantly influenced the isolation outcome. This study demonstrates that O. tsutsugamushi can often be isolated in vitro from the blood of scrub typhus patients when transported at ambient tropical temperatures for many days.
ISSN: 01251562
Appears in Collections:Scopus 2006-2010

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