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Title: Cochinin B, a novel ribosome-inactivating protein from the seeds of Momordica cochinchinensis
Authors: Juthamas Chuethong
Kohei Oda
Hiroaki Sakurai
Ikuo Saiki
Wichet Leelamanit
Mahidol University
Kyoto Institute of Technology
University of Toyama
Keywords: Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Mar-2007
Citation: Biological and Pharmaceutical Bulletin. Vol.30, No.3 (2007), 428-432
Abstract: Cochinin B, a novel ribosome-inactivating protein (RIP) with a molecular weight of 28 kDa, was purified from the seeds of Momordica cochinchinensis (Cucurbitaceae). The isolation procedure entailed ammonium sulfate precipitation, cation-exchange chromatography on SP Sepharose column and size-exclusion chromatography on Superdex 75 column with a fast protein liquid chromatography (FPLC) system. The first twenty N-terminal amino acid residues of Cochinin B showed homology to type I RIPs from other Momordica species. The purified Cochinin B displayed a strong inhibitory activity on protein synthesis in the cell-free rabbit reticulocyte lysate system with IC50of 0.36 nM. Furthermore, it exhibited N-glycosidase activity and cytotoxicity against Vero cell line with IC50higher than 1540 nM. Interestingly, Cochinin B manifested strong anti-tumor activities on human cervical epithelial carcinoma (HeLa), human embryonic kidney (HEK293) and human small cell lung cancer (NCI-H187) cell lines with IC50of 16.9, 114 and 574 nM, respectively. © 2007 Pharmaceutical Society of Japan.
ISSN: 13475215
Appears in Collections:Scopus 2006-2010

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