Simple jQuery Dropdowns
Please use this identifier to cite or link to this item:
Title: Activation of prophenoloxidase, agglutinin and antibacterial activity in haemolymph of the black tiger prawn, Penaeus monodon, by immunostimulants
Authors: K. Sritunyalucksana
P. Sithisarn
B. Withayachumnarnkul
T. W. Flegel
Mahidol University
Keywords: Agricultural and Biological Sciences;Environmental Science
Issue Date: 1-Jan-1999
Citation: Fish and Shellfish Immunology. Vol.9, No.1 (1999), 21-30
Abstract: The in vitro effect of the microbial cell wall components peptidoglycan (PG), lipopolysaccharide (LPS) and laminarin (β-1,3 glucan) on the haemolymph agglutinating and antibacterial activities were investigated in the black tiger shrimp, Penaeus monodon, using phenoloxidase (PO) activity as a reference. The effect on these factors of 3 orally administered commercial stimulants, PENSTIM, BRM-01 and BRM-02, was also determined. The study was done using shrimp haemocyte lysate fraction (HLF). It was shown that PO, agglutinating and antibacterial activities occurred naturally in shrimp haemolymph and varied considerably amongst individuals. Concentrations of PG at 0·4% and LPS at 0·002% added to HLF gave 22-fold and 11-fold increased PO activity in vitro, respectively, over the activity of HLF alone. By contrast, addition of laminarin did not increase PO activity. Attempts to stimulate agglutinating and antibacterial activity in vitro or in vivo were unsuccessful. On the contrary, 0·002% LPS was found to have an inhibitory effect on antibacterial activity. Specifically, a lower antibacterial activity was detected in samples treated with LPS after clotting (HLF), than in those treated before clotting. This suggested that LPS was involved in mechanisms for both clotting and for antibacterial activity. No relationships were found amongst PO, agglutinin and antibacterial activities in shrimp haemolymph either naturally or by activation. © 1999 Academic Press.
ISSN: 10504648
Appears in Collections:Scopus 1991-2000

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.