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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/25327
Title: Characterization of a ferric uptake regulator (fur) gene from Xanthomonas campestris pv. phaseoli with unusual primary structure, genome organization, and expression patterns
Authors: Suvit Loprasert
Ratiboot Sallabhan
Sopapan Atichartpongkul
Skorn Mongkolsuk
Chulabhorn Research Institute
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Nov-1999
Citation: Gene. Vol.239, No.2 (1999), 251-258
Abstract: A 1.5kb DNA fragment from Xanthomonas campestris pv. phaseoli containing fur was characterized. fur is a single copy gene that is transcribed as a monocistronic mRNA. The predicted amino acid sequence of Xp Fur showed extensive identity to other Fur proteins. However, Xp Fur has many distinct features, particularly a lack of cysteine residues in the conserved metal- binding motifs and unusual modifications in the carboxy-terminus region. The nucleotide sequences of fur genes from four other Xanthomonas spp. were determined. Deduced amino acid sequences all showed the distinct features of Xp Fur. Functionally, Xp Fur partially repressed a Fur-regulated promoter in E. coli. Expression analysis of fur showed increased fur mRNA levels in response to a low iron growth condition. The fur transcription start site was identified by primer extension.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0032733344&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/25327
ISSN: 03781119
Appears in Collections:Scopus 1991-2000

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