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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/25334
Title: Molecular typing of global isolates of Cryptococcus neoformans var. neoformans by polymerase chain reaction fingerprinting and randomly amplified polymorphic DNA - A pilot study to standardize techniques on which to base a detailed epidemiological survey
Authors: Wieland Meyer
Krystyna Marszewska
Mitra Amirmostofian
Ricardo Pereira Igreja
Claudia Hardtke
Katharina Methling
Maria Anna Vivianl
Ariya Chindamporn
Samaniya Sukroongreung
Melanie Ann John
David H. Ellis
Tania C. Sorrell
The University of Sydney
Universidade Federal do Rio de Janeiro
Universitat Potsdam
Universita degli Studi di Milano
Chulalongkorn University
Mahidol University
University of Natal, Faculty of Medicine
University of Adelaide
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 20-Jul-1999
Citation: Electrophoresis. Vol.20, No.8 (1999), 1790-1799
Abstract: A total of 356 clinical isolates of the encapsulated basidiomycetous fungus Cryptococcus neoformans var. neoformans, obtained from Australia, Argentina, Brazil, India, Italy, New Zealand, Papua New Guinea, South Africa, Thailand and the USA, were analyzed to lay the basis for a comprehensive evaluation of the global genetic structure of C. neoformans. Two polymerase chain reaction (PCR)-based typing techniques were standardized: PCR fingerprinting using a single primer specific to minisatellite or microsatellite DNA, and randomly amplified polymorphic DNA (RAPD) analysis using two combinations of three 20- to 22-mer random primers. Previous studies showed that the resultant profiles are reproducible and stable over time. Identical results were obtained in two different laboratories and by different scientists in the same laboratory. Both typing techniques separated the isolates into four major groups (VNI and VNII, serotype A; VNIII, serotype A/D; and VNIV, serotype D). The majority (78%) of isolates belonged to VNI, compared with 18% VNII, 1% VNIII and 3% VNIV. All US isolates could be differentiated by a unique, strain-specific PCR fingerprint or RAPD pattern in contrast to most of the non-US isolates, which showed a substantially higher degree of genetic homogeneity, with some clonality, in different parts of the world. Isolates obtained from the same patient at different times and from different body sites, had identical banding patterns. Both typing techniques should provide powerful tools for epidemiological studies of medically important fungi.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0033032335&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/25334
ISSN: 01730835
Appears in Collections:Scopus 1991-2000

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