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dc.contributor.authorWieland Meyeren_US
dc.contributor.authorKrystyna Marszewskaen_US
dc.contributor.authorMitra Amirmostofianen_US
dc.contributor.authorRicardo Pereira Igrejaen_US
dc.contributor.authorClaudia Hardtkeen_US
dc.contributor.authorKatharina Methlingen_US
dc.contributor.authorMaria Anna Vivianlen_US
dc.contributor.authorAriya Chindampornen_US
dc.contributor.authorSamaniya Sukroongreungen_US
dc.contributor.authorMelanie Ann Johnen_US
dc.contributor.authorDavid H. Ellisen_US
dc.contributor.authorTania C. Sorrellen_US
dc.contributor.otherThe University of Sydneyen_US
dc.contributor.otherUniversidade Federal do Rio de Janeiroen_US
dc.contributor.otherUniversitat Potsdamen_US
dc.contributor.otherUniversita degli Studi di Milanoen_US
dc.contributor.otherChulalongkorn Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherUniversity of Natal, Faculty of Medicineen_US
dc.contributor.otherUniversity of Adelaideen_US
dc.identifier.citationElectrophoresis. Vol.20, No.8 (1999), 1790-1799en_US
dc.description.abstractA total of 356 clinical isolates of the encapsulated basidiomycetous fungus Cryptococcus neoformans var. neoformans, obtained from Australia, Argentina, Brazil, India, Italy, New Zealand, Papua New Guinea, South Africa, Thailand and the USA, were analyzed to lay the basis for a comprehensive evaluation of the global genetic structure of C. neoformans. Two polymerase chain reaction (PCR)-based typing techniques were standardized: PCR fingerprinting using a single primer specific to minisatellite or microsatellite DNA, and randomly amplified polymorphic DNA (RAPD) analysis using two combinations of three 20- to 22-mer random primers. Previous studies showed that the resultant profiles are reproducible and stable over time. Identical results were obtained in two different laboratories and by different scientists in the same laboratory. Both typing techniques separated the isolates into four major groups (VNI and VNII, serotype A; VNIII, serotype A/D; and VNIV, serotype D). The majority (78%) of isolates belonged to VNI, compared with 18% VNII, 1% VNIII and 3% VNIV. All US isolates could be differentiated by a unique, strain-specific PCR fingerprint or RAPD pattern in contrast to most of the non-US isolates, which showed a substantially higher degree of genetic homogeneity, with some clonality, in different parts of the world. Isolates obtained from the same patient at different times and from different body sites, had identical banding patterns. Both typing techniques should provide powerful tools for epidemiological studies of medically important fungi.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleMolecular typing of global isolates of Cryptococcus neoformans var. neoformans by polymerase chain reaction fingerprinting and randomly amplified polymorphic DNA - A pilot study to standardize techniques on which to base a detailed epidemiological surveyen_US
dc.typeConference Paperen_US
Appears in Collections:Scopus 1991-2000

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