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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/25449
Title: Stable marker on flagellin gene sequences related to arabinose non- assimilating pathogenic Burkholderia pseudomallei
Authors: Sumet Wajanarogana
Piengchan Sonthayanon
Vanaporn Wuthiekanun
Sakol Panyim
Andrew J.H. Simpson
Sumalee Tungpradabkul
Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 1-Jan-1999
Citation: Microbiology and Immunology. Vol.43, No.11 (1999), 995-1001
Abstract: Using PCR-based isolation and sequence analysis of the flagellin gene from two distinct biotypes of Burkholderia pseudomallei, a 15-bp deletion was found within the variable domain of the gene in isolates capable of assimilating arabinose (Ara+). This finding led to the development of a PCR- based method in order to differentiate and identify pathogenic B. pseudomallei for epidemiological study. A pair of specific primers was designed covering the 15-bp deletion region at the variable domain. PCR- amplification products of 176 and 191 bp in size were detected from 41 Ara+ isolates and 39 Ara- isolates of B. pseudomallei, respectively. Moreover, flagellin gene fragments of other bacterial species tested in this study were not amplified using these primers. The results suggest that the flagellin gene sequences of both B. pseudomallei biotypes in this region are stable and distinct. This method can be applied and useful for the epidemiological study of B. pseudomallei.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0032724426&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/25449
ISSN: 03855600
Appears in Collections:Scopus 1991-2000

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