Please use this identifier to cite or link to this item:
http://repository.li.mahidol.ac.th/dspace/handle/123456789/25569| Title: | Cloning and characterization of a nonhemolytic phospholipase C gene from Burkholderia pseudomallei |
| Authors: | Sunee Korbsrisate Nuttiga Suwanasai Amornrut Leelaporn Takayuki Ezaki Yoshiaki Kawamura Suttipant Sarasombath Faculty of Medicine, Siriraj Hospital, Mahidol University Gifu University School of Medicine Mahidol University |
| Keywords: | Medicine |
| Issue Date: | 1-Nov-1999 |
| Citation: | Journal of Clinical Microbiology. Vol.37, No.11 (1999), 3742-3745 |
| Abstract: | We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The deduced amino acid sequence exhibited 48% similarity to that of a nonhemolytic PLC from Pseudomonas aeruginosa. The expressed PC-PLC was heat stable, nonhemolytic for sheep erythrocytes, and active between pH 2 and 8. Western blot analysis with sera from melioidosis patients indicated that they produced immunoglobulin M antibodies against this PC-PLC protein. |
| URI: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0032867180&origin=inward http://repository.li.mahidol.ac.th/dspace/handle/123456789/25569 |
| ISSN: | 00951137 |
| Appears in Collections: | Scopus 1991-2000 |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.