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Title: Cloning and characterization of a nonhemolytic phospholipase C gene from Burkholderia pseudomallei
Authors: Sunee Korbsrisate
Nuttiga Suwanasai
Amornrut Leelaporn
Takayuki Ezaki
Yoshiaki Kawamura
Suttipant Sarasombath
Faculty of Medicine, Siriraj Hospital, Mahidol University
Gifu University School of Medicine
Mahidol University
Keywords: Medicine
Issue Date: 1-Nov-1999
Citation: Journal of Clinical Microbiology. Vol.37, No.11 (1999), 3742-3745
Abstract: We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The deduced amino acid sequence exhibited 48% similarity to that of a nonhemolytic PLC from Pseudomonas aeruginosa. The expressed PC-PLC was heat stable, nonhemolytic for sheep erythrocytes, and active between pH 2 and 8. Western blot analysis with sera from melioidosis patients indicated that they produced immunoglobulin M antibodies against this PC-PLC protein.
ISSN: 00951137
Appears in Collections:Scopus 1991-2000

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