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dc.contributor.authorD. Krailasen_US
dc.contributor.authorV. Viyananten_US
dc.contributor.authorP. Ardseungnoenen_US
dc.contributor.authorP. Sobhonen_US
dc.contributor.authorE. S. Upathamen_US
dc.contributor.authorR. Keawjamen_US
dc.contributor.otherMahidol Universityen_US
dc.identifier.citationAsian Pacific Journal of Allergy and Immunology. Vol.17, No.1 (1999), 53-58en_US
dc.description.abstractWe identified three specific circulating antibodies in serum of cattle naturally infected with Fasciola gigantica. Two of the antibodies were found to react specifically to 97 and 66 kDa antigenic molecules of adult worm tegumental membrane extract. The third antibody was identified by the reaction with 26-28 kDa molecule of the excretory/secretory antigens. Monoclonal antibody against 66 kDa protein was developed and used for localization of its antigenic target in adult worm frozen sections. The experiment demonstrated that 66 kDa protein is a component on the outer surface membrane and on the membrane lining of the caecal epithelial of adult worm. The 66 kDa antigen was considered as a promising candidate for immunodiagnosis and vaccine.en_US
dc.rightsMahidol Universityen_US
dc.titleIdentification of circulating antibodies in fasciolosis and localization of 66 kDa antigenic target using monoclonal antibodiesen_US
Appears in Collections:Scopus 1991-2000

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