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Title: A comparison of lysis centrifugation, pour plate, and conventional blood culture methods in the diagnosis of septicaemic melioidosis
Authors: Andrew J.H. Simpson
P. A. Howe
V. Wuthiekanun
N. J. White
Mahidol University
Nuffield Department of Clinical Medicine
Keywords: Medicine
Issue Date: 1-Jan-1999
Citation: Journal of Clinical Pathology. Vol.52, No.8 (1999), 616-619
Abstract: Aims - To determine whether quantitative blood culture methods could improve the diagnosis of septicaemic melioidosis. Methods - A comparison of conventional broth based blood cultures, a pour plate method, and a commercial lysis centrifugation (Isolator 10(TM) blood culture system was conducted in 71 Thai patients with severe melioidosis. The time to identification of B pseudomallei was recorded for each method. Results - 42 patients (59%) were septicaemic. Compared with conventional blood culture, the Isolator and pour plate methods had sensitivities of 81% and 61%, respectively. The median times to a positive culture were: Isolator 39.3 hours, pour plates 45.5 hours, broth culture 61.8 hours (p < 0.001 Isolator υ broth). There was a significant inverse correlation between Isolator tube or pour plate quantitative counts and time to detection (r = -0.44 and -0.57, respectively). Mortality was higher in patients who were septicaemic. Conclusions - Routine use of one of these quantitative methods, in addition to conventional broth culture, may lead to earlier diagnosis of septicaemic melioidosis.
ISSN: 00219746
Appears in Collections:Scopus 1991-2000

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