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dc.contributor.authorAndrew J.H. Simpsonen_US
dc.contributor.authorP. A. Hoween_US
dc.contributor.authorV. Wuthiekanunen_US
dc.contributor.authorN. J. Whiteen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherNuffield Department of Clinical Medicineen_US
dc.date.accessioned2018-09-07T09:00:55Z-
dc.date.available2018-09-07T09:00:55Z-
dc.date.issued1999-01-01en_US
dc.identifier.citationJournal of Clinical Pathology. Vol.52, No.8 (1999), 616-619en_US
dc.identifier.issn00219746en_US
dc.identifier.other2-s2.0-0032809083en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0032809083&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/25757-
dc.description.abstractAims - To determine whether quantitative blood culture methods could improve the diagnosis of septicaemic melioidosis. Methods - A comparison of conventional broth based blood cultures, a pour plate method, and a commercial lysis centrifugation (Isolator 10(TM) blood culture system was conducted in 71 Thai patients with severe melioidosis. The time to identification of B pseudomallei was recorded for each method. Results - 42 patients (59%) were septicaemic. Compared with conventional blood culture, the Isolator and pour plate methods had sensitivities of 81% and 61%, respectively. The median times to a positive culture were: Isolator 39.3 hours, pour plates 45.5 hours, broth culture 61.8 hours (p < 0.001 Isolator υ broth). There was a significant inverse correlation between Isolator tube or pour plate quantitative counts and time to detection (r = -0.44 and -0.57, respectively). Mortality was higher in patients who were septicaemic. Conclusions - Routine use of one of these quantitative methods, in addition to conventional broth culture, may lead to earlier diagnosis of septicaemic melioidosis.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0032809083&origin=inwarden_US
dc.subjectMedicineen_US
dc.titleA comparison of lysis centrifugation, pour plate, and conventional blood culture methods in the diagnosis of septicaemic melioidosisen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1136/jcp.52.8.616en_US
Appears in Collections:Scopus 1991-2000

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