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Title: High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant
Authors: W. Panbangred
K. Weeradechapon
S. Udomvaraphant
K. Fujiyama
V. Meevootisom
Mahidol University
Osaka University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 15-Aug-2000
Citation: Journal of Applied Microbiology. Vol.89, No.1 (2000), 152-157
Abstract: By marker exchange mutagenesis, Bacillus megaterium strain UN-1 (Bm-UN1) was used to prepare a mutant strain B. megaterium UN-cat (Bm-UNcat) lacking the penicillin G acylase gene (pac). The pac gene from Bm-UN1 was subcloned into pTF6 and the resultant plasmid, pBA402, was introduced into Bm-UNcat and Bacillus subtilis. Bm-UNcat harbouring pBA402 produced high penicillin G acylase (PAC) activity of 13.7, 19.5 and 20.4 U ml1 at 24, 36 and 48 h of culture, respectively. This was two- to fivefold higher than PAC produced by B. subtilis harbouring pBA402 and about 20-fold higher than PAC produced by the parent strain, Bm-UN1.
ISSN: 13645072
Appears in Collections:Scopus 1991-2000

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