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Title: Lymphocyte subsets and specific T-cell immune response in thalassemia
Authors: Kovit Pattanapanyasat
Charin Thepthai
Pornvaree Lamchiagdhase
Surada Lerdwana
Kalaya Tachavanich
Prayoon Thanomsuk
Wanchai Wanachiwanawin
Suthat Fucharoen
Janice M. Darden
Mahidol University
Faculty of Medicine, Siriraj Hospital, Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 15-Feb-2000
Citation: Communications in Clinical Cytometry. Vol.42, No.1 (2000), 11-17
Abstract: Infection is very common in thalassemia and is one of the major causes of death. To date, it is not quite clear why these patients are susceptible to infection. In this study, lymphocyte immunophenotyping for CD3+ (T- cells), CD3+CD4+ (T-helper/inducer cells), CD3+CD8+ (T- suppressor/cytotoxic cells), CD3-CD19+ (B-cells), and CD3-CD16/56+ (natural killer cells) subsets and expression of the activation antigen CD69 on CD3+CD4+ and CD3+CD8+ T-cells were determined in the whole blood of thalassemia patients, using a three-color flow cytometric technique. Results showed that only splenectomized β-thalassemia/hemoglobin (Hb) E patients displayed a marked increase in absolute number of all lymphocytes. In addition, splenectomized β-thalassemia/Hb E showed a significantly lower percentage of CD3+ cells, with a corresponding increase in CD19+ cells. These differences, when compared with normal subjects and other thalassemia patients, may be attributed to splenectomy, α-thalassemia patients, on the other hand, showed no significant difference from the normal group. While lymphocyte subsets in splenectomized β-thalassemia/Hb E patients showed an abnormal distribution, T-cell activation in these patients was not different from the activation seen in normal subjects. This implies that thalassemia patients, during the steady state of disease, appear to have normal T- lymphocyte function with only moderate abnormalities of T- and B-lymphocyte subsets. (C) 2000 Wiley-Liss, Inc.
ISSN: 01964763
Appears in Collections:Scopus 1991-2000

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